Genome-wide double-stranded RNA sequencing reveals the functional significance of base-paired RNAs in Arabidopsis

ABSTRACT: The functional structure of all biologically active molecules is dependent on intra- and inter-molecular interactions. This is especially evident for RNA molecules whose functionality, maturation, and regulation requires formation of correct secondary structure through encoded base-pairing interactions. Unfortunately, intra- and inter-molecular base-pairing information is lacking for most RNAs. Here, we use high-throughput sequencing to interrogate all base-paired RNA in Arabidopsis thaliana, and identify ~200 new small (sm)RNA-producing substrates of RNA-DEPENDENT RNA POLYMERASE 6. Our comprehensive analysis of paired RNAs reveals conserved functionality within introns and both 5’ and 3’ untranslated regions (UTRs) of mRNAs, as well as a novel population of functional RNAs, many of which

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Qi Zheng 

PROVIDER: E-GEOD-23439 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress