The IHF regulon of exponentially growing Pseudomonas putida cells

ABSTRACT: Integration host factor (IHF) sites are largely absent from intergenic regions of ORFs encoding central metabolic functions in Pseudomonas putida mt-2. To gain an insight into this unequal distribution of otherwise abundant IHF-binding sequences, the transcriptome of IHF-plus and IHF-minus cells growing exponentially on glucose as sole carbon source was examined. In parallel, the cognate metabolic fluxes of the wild-type P. putida strain and its ihfA derivative were determined by culturing cells to a steady-state physiological regime with (13) C-labelled glucose. While expression of many transcripts was altered by the lack of IHF, flux balance analysis revealed that the ihfA mutation did not influence central carbon metabolism. Identification of multiple IHF sites adjacent to genes respons

ORGANISM(S): Pseudomonas putida

SUBMITTER: Victor de Lorenzo 

PROVIDER: E-GEOD-23499 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress