Identification and expression analysis of differentially expressed genes in a hepatocyte model of cold-induced glycerol production in rainbow smelt (Osmerus mordax)

ABSTRACT: The rainbow smelt (Osmerus mordax, Mitchill, 1814) is an anadromous teleost that overwinters in the estuaries and inshore waters along the North American Atlantic coastline. In the winter months, smelt avoid freezing in subzero temperatures by the production of antifreeze proteins and high levels of glycerol. Glycerol production (glyceroneogenesis) occurs in the liver via a branch point in glycolysis and gluconeogenesis and is directly activated by low temperature. In these studies, hepatocytes were isolated from the liver of individual warm fish and incubated at either a warm (8ºC; non-glycerol accumulating) or cold (0.4ºC; glycerol accumulating) temperature over a 72 h time course. Functional genomic techniques were used to identify and validate hepatic transcripts that were differential

ORGANISM(S): Osmerus mordax

SUBMITTER: Jennifer Hall 

PROVIDER: E-GEOD-23516 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress