Pol II (Rpb3) ChIP-seq in Drosophila melanogaster S2 cells

ABSTRACT: Control of RNA transcription is critical for the development and homeostasis of all organisms, and can occur at multiple steps of the transcription cycle, including RNA polymerase II (Pol II) recruitment, initiation, promoter-proximal pausing, and elongation. That Pol II accumulates on many promoters in metazoans implies that steps other than Pol II recruitment are rate-limiting and regulated 1-6. By integrating genome-wide Pol II chromatin immunoprecipition (ChIP) and Global Run-On (GRO) genomic data sets from Drosophila cells, we examined critical features of Pol II near promoters. The accumulation of promoter-proximal polymerase is widespread, occurring on 70% of active genes; and unlike elongating Pol II within the body of genes, promoter Pol II are held paused by factors like NELF, u

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Leighton Core 

PROVIDER: E-GEOD-23542 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress