Analysis of FoxA2 occupancy in mouse liver with endoderm ChIP-chip array

ABSTRACT: Understanding how silent genes can be competent for activation provides insight into development as well as cellular reprogramming and pathogenesis. We performed genomic location analysis of the pioneer transcription factor FoxA in the adult liver and found that about one-third of the FoxA bound sites are near silent genes, including genes without detectable RNA polymerase II. Virtually all of the FoxA-bound silent sites are within extended conserved sequences, suggesting possible function. Such sequences are enriched in motifs for transcriptional repressors, including for Rfx1. We found one such target site containing functional FoxA and Rfx1 sites is a cryptic enhancer 7 kb downstream of the Cdx2 gene, the latter being an effector of esophageal metaplasia. Indeed, Rfx1 sites restric

ORGANISM(S): Mus musculus

SUBMITTER: Gregory Donahue 

PROVIDER: E-GEOD-23551 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress