Salmonella_14028s - Trancriptomic analysis in Salmonella typhimurium infection
Ontology highlight
ABSTRACT: au10-06_salmonella_eduardob - salmonella_14028s - Transcriptomic analysis between Salmonella wt and differents mutants in type3 secretion system - Plants were grown during 14 days in solid medium and were transfered to liquid medium during 16 h, at 9 am samonella was added to a final concentration of OD600=0.2. Keywords: normal vs disease comparison 2 dye-swap - CATMA arrays
Project description:au10-06_salmonella_eduardob - inva_vs_14028s Transcriptomic analysis between Salmonella wt and different mutants in type3 secretion system. - Plants were grown during 14 days in solid medium and were transfered to liquid medium during 16 h. at 9 am samonella was added to a final concentration of OD600=0.2 2 dye-swap - normal vs disease comparison
Project description:au10-01_overexpressionoxi1 - au10-01_overexpressionoxi1 - Analysis of the overexpression of the Oxi1 - Comparision between the overexpression of oxi1 and wt. 2 dye-swap - normal vs transgenic comparison
Project description:rs11-10_tut1 - leaf transcriptome comparison between wt and tut1 (salk_087647) - Identification of mRNA targets of the terminal uridylyltransferase TUT1 (AT2G45620) in Arabidopsis thaliana. - Identification of the RNA targets of the terminal uridylyl transferase TUT1 (AT2G45620). Mature rosettte leaf transcriptomes were compared between WT and a tut1 knock-out mutant (SALK_087647). 3 dye-swap - gene knock out
Project description:cea05-01_carbonic-anhydrase - carbonic anhydrase (1 or 2) simple or double mutants - Identification of genes differentially expressed in leaves of single CA1 and CA2 T-DNA insertional mutants and in the corresponding double mutant vs wild type - CA1 (At3g01500) and CA2 (At5g14740) T-DNA insertional mutant lines, the double (CA1+CA2) mutant and wild type Arabidopsis seeds were sown in soil in a phytotron. Leaves were harvested 40 days later for RNA extraction 6 dye-swap - gene knock out
Project description:au10-06_salmonella_eduardob - salmonella_prgh - Transcriptomic analysis between Salmonella wt and differents mutants in type3 secretion system - Plants were grown during 14 days in solid medium and were transfered to liquid medium during 16 h, at 9 am samonella was added to a final concentration of OD600=0.2. Keywords: normal vs disease comparison 2 dye-swap - CATMA arrays
Project description:au10-06_salmonella - salmonell inva - Transcriptomic analysis between Salmonella wt and differents mutants in type3 secretion system. - Plants were grown during 14 days in solid medium and were transfered to liquid medium during 16 h. at 9 am samonella was added to a final concentration of OD600=0.2 2 dye-swap - normal vs antisense mutant comparison
Project description:The mkkkC5 mutant, a knockout line of MAPKKKC5, shows changes in growth and development, as well as an altered Alternaria sensitivity. It was shown by Brader and Djamei et al.(2007) that Alternaria brassicicola sensitivity is also influenced by MKK2-activity. Aim of this experiment is to access the transcriptional changes in the mkkkc5plants as well as in plants overexpressing a constitutively active MKK2-version (MKK2EE). The second part of the experiment addresses transcriptional changes in these plants after Alternaria brassicicola infection. au07-09_alternaria Keywords: treatment variations Alternaria brassicicola infection of 25 day old mkkkc5- , pGreen::MKK2EE-myc and WT Col-0 plants. Sampling after 0, 1 and 2 days. Comparison between mutants and WT at corresponding timepoints. Additionally comparison between WT-samples at different timepoints. 16 dye-swap - CATMA arrays
Project description:The mkkkC5 mutant, a knockout line of MAPKKKC5, shows changes in growth and development, as well as an altered Alternaria sensitivity. It was shown by Brader and Djamei et al.(2007) that Alternaria brassicicola sensitivity is also influenced by MKK2-activity. Aim of this experiment is to access the transcriptional changes in the mkkkc5plants as well as in plants overexpressing a constitutively active MKK2-version (MKK2EE). The second part of the experiment addresses transcriptional changes in these plants after Alternaria brassicicola infection. - au07-09_alternaria - mkk2ee and mkkkc5 mutants Keywords: wt vs mutant comparison Comparison between the over-expressed mutant of the MKK2 gene and the KO mutant for the MKKKC5 gene versus the wild type. 4 dye-swap - CATMA arrays