Genome-wide analysis of insulin-like growth factor 1 receptor (IGF1R) binding sites in DFB cells

ABSTRACT: The insulin-like growth factor 1 receptor (IGF-1R) plays crucial roles in developmental and cancer biology. Most of its biological effects have been ascribed to its tyrosine kinase activity. We report that IGF-1 promotes the modification of IGF-1R by small ubiquitin-like modifier protein-1 (SUMO-1) and its translocation to the nucleus. Nuclear IGF-1R associated with enhancer-like elements and increased transcription in reporter assays. We used ChIP-seq to examine the interaction of IGF-1R with DNA on a genome-wide scale. Analysis of the data set resulted in 568 candidate peaks, that is, statistically significant IGF-1R-enriched regions. The IGF-1R-enriched regions were divided into five classes on the basis of their location relative to known genes. Most of the IGF-1R-interacting sites (80

ORGANISM(S): Homo sapiens

SUBMITTER: Ali Tofigh 

PROVIDER: E-GEOD-23792 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress