TEL-AML1 regulation of survivin and apoptosis via miRNA-494 and miRNA-320a

ABSTRACT: There is increasing evidence that microRNA and transcription factors interact in an instructive fashion in normal and malignant hematopoiesis. We explored the impact of TEL-AML1 (ETV6-RUNX1), the most common fusion protein in childhood leukemia, on miRNA expression and the leukemic phenotype. Using RNA interference, miRNA expression arrays, and quantitative PCR, we identified miRNA-494 and miRNA-320a to be upregulated upon TEL-AML1 silencing independently of TEL expression. Chromatin immunoprecipitation analysis identified miRNA-494 as a direct miRNA target of the fusion protein TEL-AML1. Using bioinformatic analysis as well as functional luciferase experiments we demonstrate that survivin is a target of the two miRNAs. MiRNA-494 and -320a were introduced to the cells by transfecti

ORGANISM(S): Homo sapiens

SUBMITTER: Joseph Wiemels 

PROVIDER: E-GEOD-23842 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress