Transcription profiling of human normal and cadaver primary open-angle glaucoma donors

ABSTRACT: The total RNA from control and POAG optic nerve lamina cribrosa region was isolated and subjected to analysis on U133A 2.0 affymetrix microarray chip. The results from these two analysis was compared to determine whether PAD II RNA level is altered. Purpose of the study. This study was done to determine which RNA transcripts undergo significant changes in the optic nerve between normal and cadaver primary open-angle glaucoma donors. Using donor tissues from NDRI we have performed proteomic analyses (which will be published shortly), we intend a comparison between the proteomic and RNA changes for select proteins in our study. Sample Collection and Preparation of Labeled Copy RNA; The total RNA from optic nerve was obtained using TRIZOL (Invitrogen Inc., Carlsbad, CA) with modification of

ORGANISM(S): Homo sapiens

SUBMITTER: Sanjoy Bhattacharya 

PROVIDER: E-GEOD-2387 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress