Temporal uncoupling of the DNA methylome and transcriptional repression during embryogenesis

ABSTRACT: DNA methylation is a tightly regulated epigenetic mark associated with transcriptional repression. Next-generation sequencing of purified methylated DNA obtained from early Xenopus tropicalis embryos demonstrates that this genome is heavily methylated during blastula and gastrula stages. Although DNA methylation is largely absent from transcriptional start sites marked with histone H3 lysine 4 trimethylation (H3K4me3), we find both promoters and gene bodies of active genes robustly methylated. By contrast, DNA methylation is absent in large H3K27me3 domains, indicating these two repression pathways have different roles. Comparison with chromatin state maps of human ES cells reveals strong conservation of epigenetic makeup and gene regulation between the two systems. Strikingly, genes that

ORGANISM(S): Xenopus (Silurana) tropicalis

SUBMITTER: Ozren Bogdanovic 

PROVIDER: E-GEOD-23913 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress