Gene expression study identifies important protective pathways utilized by appendicitis and appendectomy for ameliorating colitis

ABSTRACT: BACKGROUND: Appendicitis followed by appendectomy (AA) at a young age protects against later inflammatory bowel disease (IBD). Using a novel murine appendicitis model we earlier demonstrated that AA proffered significant protection against subsequent experimental colitis. AIM: To delineate genes and biological pathways involved in the protective effect of AA against subsequent colitis using gene set enrichment analysis (GSEA) of DNA microarray data. METHODS: Appendicitis and appendicectomy was done (5 week old male BALB/c mice) near the most proximal colon (caecal lymphoid follicles) and colonic samples were harvested from the most distal colon. Two consecutive laparotomies were done in control Sham-Sham (SS) mice. RNA was extracted (TRIzol®) from 4 individual colonic samples per group (A

ORGANISM(S): Mus musculus

SUBMITTER: Rajkumar Cheluvappa 

PROVIDER: E-GEOD-23914 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress