Budding yeast RNA polymerase II CTD phosphorylation and transcription termination factor localization
Ontology highlight
ABSTRACT:
ORGANISM(S): Saccharomyces cerevisiae
SUBMITTER: David Bentley
PROVIDER: E-GEOD-23960 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Gene-specific RNA polymerase II phosphorylation and the CTD code.
Kim Hyunmin H Erickson Benjamin B Luo Weifei W Seward David D Graber Joel H JH Pollock David D DD Megee Paul C PC Bentley David L DL
Nature structural & molecular biology 20100912 10
Phosphorylation of the RNA polymerase (Pol) II C-terminal domain (CTD) repeats (1-YSPTSPS-7) is coupled to transcription and may act as a 'code' that controls mRNA synthesis and processing. To examine the code in budding yeast, we mapped genome-wide CTD Ser2, Ser5 and Ser7 phosphorylations and the CTD-associated termination factors Nrd1 and Pcf11. Phospho-CTD dynamics are not scaled to gene length and are gene-specific, with highest Ser5 and Ser7 phosphorylation at the 5' ends of well-expressed ...[more]
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