Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

13 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Hepatic Gene Expression Profiling of 5'AMP Induced Hypometabolism in Mice

ABSTRACT: Mice injected IP with 5'-AMP enter a hypometabolic state that is an induced hybernation (AIHM). Recovery from this AIHM is marked by reverse flip (RF) behavior Illumina arrays were used to profile control, AIHM and RF aminals in RNA prepared from the liver. Animals were injected with vehicle or 0.5 mg/g body weight 5'-adenosine monophosphate. At 6 hours (AIHM) or 12 hours (RF) animals were killed and total RNA prepared from the liver.

ORGANISM(S): Mus musculus

SUBMITTER: David Loose

PROVIDER: E-GEOD-23975 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Hepatic gene expression profiling of 5'-AMP-induced hypometabolism in mice.

Zhao Zhaoyang Z Miki Takao T Van Oort-Jansen Anita A Matsumoto Tomoko T Loose David S DS Lee Cheng Chi CC

Physiological genomics 20110111 7

There is currently much interest in clinical applications of therapeutic hypothermia. Hypothermia can be a consequence of hypometabolism. We have recently established a procedure for the induction of a reversible deep hypometabolic state in mice using 5'-adenosine monophosphate (5'-AMP) in conjunction with moderate ambient temperature. The current study aims at investigating the impact of this technology at the gene expression level in a major metabolic organ, the liver. Our findings reveal that ...[more]

PMID: 21224422

Similar Datasets

Hepatic Gene Expression Profiling of 5'AMP Induced Hypometabolism in Mice

Project description:Mice injected IP with 5'-AMP enter a hypometabolic state that is an induced hybernation (AIHM). Recovery from this AIHM is marked by reverse flip (RF) behavior Illumina arrays were used to profile control, AIHM and RF aminals in RNA prepared from the liver.

2012-06-20 | GSE23975 | GEO

Genome-wide screening reveals metabolic regulation of translational fidelity by cyclic AMP

Project description:Translational quality control is critical for maintaining the accuracy of protein synthesis in all domains of life. Mutations in aminoacyl-tRNA synthetases and the ribosome are known to affect translational fidelity and alter fitness, viability, stress responses, neuron function, and life span. In this study, we used a high-throughput fluorescence-based assay to screen a knock-out library of Escherichia coli and identified 30 nonessential genes that are critical for maintaining the fidelity of stop-codon readthrough. Most of these identified genes have not been shown to affect translational fidelity previously. Intriguingly, we show that several genes controlling metabolism, including cyaA and guaA, unexpectedly enhance stop-codon readthrough. CyaA and GuaA catalyze the synthesis of cyclic adenosine monophosphate (cAMP) and guanosine monophosphate (GMP), respectively. Both CyaA and GuaA increase the expression of ribosomes and tRNAs, allowing aminoacyl-tRNAs to compete with release factors and suppress stop codons. In addition, the effect of guaA deletion on stop-codon readthrough is abolished by deleting prfC, which encodes release factor 3 (RF3). Our results suggest that nucleotide and carbon metabolism is tightly coupled with translational fidelity.

2023-03-28 | GSE228051 | GEO

Structural basis of chaperone regulation by a post-translational modification

Project description:The Hsp70 chaperone BiP is covalently modified with adenosine monophosphate (referred to as AMPylation) in order to adapt its activity to the fluctuating folding load within the endoplasmic reticulum. This modification is catalyzed by the only human representative of the family of filamentation induced by cyclic adenosine monophosphate (Fic) enzymes HYPE/FICD. The structural basis for BiP binding and AMPylation has remained elusive due to the low affinity of enzyme substrate complexes.

2021-04-27 | PXD022869 | Pride

Transcription profiling by array of rat cerebellar granular neuronal precursor cells plated with Sonic Hedgehog (Shh) and then treated with the PKA activator DBA

Project description:Cerebellar granular neuronal precursors were plated in presence of Sonic Hedgehog (Shh) for 24h and then treated with the PKA activator Dibutyryl Cyclic Adenosine Monophosphate (DBA) for addittional 24h

2008-07-11 | E-MEXP-1668 | biostudies-arrayexpress

RNASeq of human bronchial epithelial (HBE) cells with cGAS-siRNA transfected

Project description:Cyclic guanosine monophosphate (GMP)-adenosine monophosphate (AMP) synthase (cGAS) is a newly discovered sensor that detects cytosolic DNA as a universal danger-associated molecular pattern (DAMP). Here, we used RNA-seq to analyze transcriptomic expression difference between control-siRNA and cGAS-siRNA cells to find out what genes involved in the regulation of inflammation and injury.

2022-10-01 | GSE185523 | GEO

Growth of Staphylococcus in the absence of c-di-AMP

Project description:Cyclic-di-adenosine monophosphate (c-di-AMP) is required for osmotic regulation in Staphylococcus aureus but dispensable for viability in anaerobic conditions

| PRJEB22312 | ENA

Effect of OVO-like 1 knockdown on global transcript expression in differentiated BeWo trophoblast cells

Project description:We had previously discovered that the transcription factor OVO-like 1 (OVOL1) was highly induced during trophoblast differentiation. In this study, we used an lentiviral shRNA strategy to decrease OVOL1 expression in BeWo trophoblast cells. Control cells were transduced with shRNAs targeting no known mammalian transcript (shCont). Following stimulation of differentiation (48h exposure to 8-bromo-cyclic adenosine monophosphate), a RNA-seq approach was used to determine global transcript differences in OVOL1-knockdown cells compared to control cells. Trophoblast cells transduced with control shRNAs were used as controls. Cells transduced with shRNAs targeting OVOL1 were used as treatment. All cells received 250 uM 8-bromo-cyclic adenosine monophosphate to stimulate differentiation. Three independent replicates of control and treatment groups were analyzed.

2015-08-01 | E-GEOD-66962 | biostudies-arrayexpress

c-di-AMP Metabolism in S. aureus

Project description:New Insights into the Cyclic Di-adenosine Monophosphate (c-di-AMP) Degradation Pathway and the Requirement of the Cyclic Dinucleotide for Acid Stress Resistance in Staphylococcus aureus

| PRJEB14723 | ENA

Edema Toxin-treated Macrophage Study

Project description:Edema toxin (EdTx), which is a combination of edema factor and a binding moiety (protective antigen), is produced by Bacillus anthracis, the etiological agent of anthrax. EdTx is an adenylyl cyclase enzyme that converts adenosine triphosphate to adenosine-3’,5’-monophosphate, resulting in interstitial edema seen in anthrax patients. We used GeneChip analysis to examine global transcriptional profiles of EdTx-treated RAW 264.7 murine macrophage-like cells at 3 and 6 hr. Keywords: Toxin response

2006-10-24 | GSE4712 | GEO

Gene expression in undifferentiated or cyclic adenosine monophosphate-exposed BeWo trophoblast cells

Project description:BeWo trophoblast cells differentiate in response to expsure to cyclic adenosine monophosphate (cAMP) analogs. Differentiation includes syncytialization (fusion) and hormonogenesis. The goal of this study was to globally determine transcripts differentially expressed in BeWo trophoblast cells following a 24-h exposure to 250 uM 8-bromo-cAMP.

2015-08-01 | GSE66840 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data