Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

MiRNA profiling of HPCX1 linked prostate cancer patients and their healthy brothers

ABSTRACT: MicroRNA expression levels in the lymphoblastic cells of prostate cancer patients and their healthy brothers from HPCX1 linked prostate cancer families were analyzed to trace variants that might alter miRNA expression and explain partly an inherited genetic predisposion to prostate cancer. MicroRNA expression levels in lymphoblastoid cell lines were detected using an Agilent Human miRNA V2 Oligo Microarray Kit (Agilent Technologies).100 ng of total RNA was used as a starting material, and miRNAs were labeled using the Agilent miRNA Labeling Kit. Labeled RNA was hybridized to Agilent miRNA arrays with eight identical arrays per slide, with each array containing probes directed against 723 human and 76 human viral miRNAs. Slides were scanned (Agilent microarray scanner) after hybridization and data was extracted using Feature Extraction software, version 9.5.1. (Agilent Technologies). Altogether 14 cancer patients and 15 healthy brothers from 11 families were included with no replicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Henna Mattila

PROVIDER: E-GEOD-24201 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

NMD and microRNA expression profiling of the HPCX1 locus reveal MAGEC1 as a candidate prostate cancer predisposition gene.

Mattila Henna H Schindler Martin M Isotalo Jarkko J Ikonen Tarja T Vihinen Mauno M Oja Hannu H Tammela Teuvo L J TL Wahlfors Tiina T Schleutker Johanna J

BMC cancer 20110802

<h4>Background</h4>Several predisposition loci for hereditary prostate cancer (HPC) have been suggested, including HPCX1 at Xq27-q28, but due to the complex structure of the region, the susceptibility gene has not yet been identified.<h4>Methods</h4>In this study, nonsense-mediated mRNA decay (NMD) inhibition was used for the discovery of truncating mutations. Six prostate cancer (PC) patients and their healthy brothers were selected from a group of HPCX1-linked families. Expression analyses wer ...[more]

PMID: 21810217

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Dataset Information

MiRNA profiling of HPCX1 linked prostate cancer patients and their healthy brothers

Publications

NMD and microRNA expression profiling of the HPCX1 locus reveal MAGEC1 as a candidate prostate cancer predisposition gene.

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