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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide H3K9 acetylation state before and after VPA treatment

ABSTRACT: Genome-wide maps of the H3K9 acetylation state in embryonic stem cells (ESCs) before and after treatment with low levels of the histone deacetylase (HDAC) inhibitor valproic acid (VPA). ChIP-seq for 3 samples: untreated E14 cells, cells treated with VPA for 4 hrs and cells treated with VPA for 16 hrs. Unprecipitated DNA was used as the input control (Input).

ORGANISM(S): Mus musculus

SUBMITTER: Eran Meshorer

PROVIDER: E-GEOD-24211 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Pluripotency-related, valproic acid (VPA)-induced genome-wide histone H3 lysine 9 (H3K9) acetylation patterns in embryonic stem cells.

Hezroni Hadas H Sailaja Badi Sri BS Meshorer Eran E

The Journal of biological chemistry 20110817 41

Embryonic stem cell (ESC) chromatin is characterized by a unique set of histone modifications, including enrichment for H3 lysine 9 acetylation (H3K9ac). Recent studies suggest that histone deacetylase (HDAC) inhibitors promote pluripotency. Here, using H3K9ac ChIP followed by high throughput sequencing analyses and gene expression in E14 mouse ESCs before and after treatment with a low level of the HDAC inhibitor valproic acid, we show that H3K9ac is enriched at gene promoters and is highly cor ...[more]

PMID: 21849501

Publication: 1/2

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