Insulin resistance induced by physical inactivity is associated with multiple transcriptional changes in skeletal muscle in young men

ABSTRACT: Rationale: Physical inactivity is a risk factor for insulin resistance. We examined the effect of nine days of bed rest on basal and insulin stimulated expression of genes potentially involved in insulin action by applying hypothesis-generating microarray in parallel with candidate gene real-time PCR approaches in 20 healthy, young men. Furthermore, we investigated whether bed rest affected DNA methylation in the promoter region of the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PPARGC1A) gene. Subjects were re-examined after four weeks of retraining. Findings: Bed rest induced insulin resistance and altered the expression of more than 4,500 genes. These changes were only partly normalized after four weeks of retraining. Pathway analyses revealed significant down-

ORGANISM(S): Homo sapiens

SUBMITTER: Natalie Hiscock 

PROVIDER: E-GEOD-24215 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress