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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Deep transcriptional sequencing analysis of human prostate adenocarcinoma and reference samples

ABSTRACT: Prostate adenocarcinoma and matched adjacent normal samples were profiled by deep transcriptional sequencing to analyze transcription-induced chimeras and gene fusions. Reference samples from the MAQC and brain and universal reference libraries were also sequenced. Two-condition experiment plus reference samples: Prostate adenocarcinoma versus matched normal from three separate patients, plus brain and universal reference samples from the MAQC project.

ORGANISM(S): Homo sapiens

SUBMITTER: Thomas Wu

PROVIDER: E-GEOD-24283 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deep RNA sequencing analysis of readthrough gene fusions in human prostate adenocarcinoma and reference samples.

Nacu Serban S Yuan Wenlin W Kan Zhengyan Z Bhatt Deepali D Rivers Celina Sanchez CS Stinson Jeremy J Peters Brock A BA Modrusan Zora Z Jung Kenneth K Seshagiri Somasekar S Wu Thomas D TD

BMC medical genomics 20110124

<h4>Background</h4>Readthrough fusions across adjacent genes in the genome, or transcription-induced chimeras (TICs), have been estimated using expressed sequence tag (EST) libraries to involve 4-6% of all genes. Deep transcriptional sequencing (RNA-Seq) now makes it possible to study the occurrence and expression levels of TICs in individual samples across the genome.<h4>Methods</h4>We performed single-end RNA-Seq on three human prostate adenocarcinoma samples and their corresponding normal tis ...[more]

PMID: 21261984

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