UC San Diego_Friedmann_Kang_Human fibroblast knockdown

ABSTRACT: To characterize mechanisms responsible for the CNS dopamine deficiency and the resulting neuropathology caused by deficiency of the housekeeping purine salvage function hypoxanthine guanine phospho- ribosyltransferase (HPRT) in the Lesch Nyhan Disease (LND), we have used microarray-based methods of global gene expression together with quantitative PCR and Western blot analysis to identify dysregulation of genes and aberrant cellular processes in human fibroblasts and in SH-SY5Y neuroblastoma cells made HPRT-deficient by transduction with a retrovirus stably expressing an shRNA targeted against HPRT. Analysis of the microarray expression data by Gene ontology (GO) and Gene Set Enrichment Analysis (GSEA) as well as by GeneSpring GX10 and Panther Classification System reveal that HPRT deficie

ORGANISM(S): Homo sapiens

SUBMITTER: Tae Hyuk Kang 

PROVIDER: E-GEOD-24345 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress