Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Global Analysis of the Transcriptional Response of Whitefly to Tomato Yellow Leaf Curl China Virus (TYLCCNV)

ABSTRACT: We investigated the transcriptional response of invasive B. tabaci B biotype to tomato yellow leaf curl China virus (TYLCCNV) using Illumina sequencing technology. We found that 1,606 genes involved in 157 biochemical pathways were differentially expressed in the viruliferous whiteflies. Culture of B biotype whitefly was maintained on cotton plants. Three thousands of newly emerged adults of whitefly on cotton were released onto the leaves of healthy and viruliferous tobacco plants. They were allowed to feed for 24 h. After that, non-viruliferous and viruliferous whiteflies were transferred respectively to cotton plants in different cages and allowed to feed for 120 h. Then approximately 1,000 non-viruliferous and viruliferous female adults of whitefly were collected, respectively. The RNA was extracted and sequenced using Illunima Analyzer II.

ORGANISM(S): Bemisia tabaci

SUBMITTER: Xiaowei Wang 

PROVIDER: E-GEOD-24374 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global analysis of the transcriptional response of whitefly to tomato yellow leaf curl China virus reveals the relationship of coevolved adaptations.

Luan Jun-Bo JB   Li Jun-Min JM   Varela Nélia N   Wang Yong-Liang YL   Li Fang-Fang FF   Bao Yan-Yuan YY   Zhang Chuan-Xi CX   Liu Shu-Sheng SS   Wang Xiao-Wei XW  

Journal of virology 20110126 7

The begomoviruses are the largest and most economically important group of plant viruses transmitted exclusively by the whitefly Bemisia tabaci in a circulative, persistent manner. The circulation of the viruses within the insect vectors involves complex interactions between virus and vector components; however, the molecular mechanisms of these interactions remain largely unknown. Here we investigated the transcriptional response of the invasive B. tabaci Middle East-Asia Minor 1 species to Tom  ...[more]

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