Time course of Streptomyces coelicolor: M145 Control vs. wblA deletion mutant

ABSTRACT: The basic experiment is a comparison of gene transcript levels of Streptomyces coelicolor M145 and its wblA deletion mutant at 6 timepoints (24, 36, 48, 60, 72 and 84 hours) encompassing vegetative growth, aerial growth, and sporulation on solid medium. The wblA mutant is morphologically defective, most of its aerial hyphae failing to show any sign of sporulation-directed attributes, and also overproduces some antibiotics. The microarray analysis was aimed to reveal the major transcriptional changes underpinning or associated with this pleiotropic phenotypic change. Using a fairly stringent cut-off, 291 genes were found to be affected, including developmental genes, antibiotic biosynthetic genes, and genes for primary metabolism. Some genes were over-expressed and others under-expressed in

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Govind Chandra 

PROVIDER: E-GEOD-24396 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress