Evidence of Distinct Tumor Initiating Cells with Different Properties in Primary Human Hepatocellular Carcinoma.

ABSTRACT: Increasing evidences have been recently reported on tumor cells heterogeneity within several malignancies, but data on liver tumours are still lacking. The aim of this study was to investigate and characterize tumor initiating cell (TIC) compartments within human hepatocellular carcinoma (HCC). After long term culture, we were able to identify 3 morphologically different tumor cell populations from an individual HCC specimen. These cell populations were extensively characterized by flow cytometry, fluorescence microscopy, single cell cloning, xenotransplantation in NOD/SCID IL2Rgamma -/- mice, karyotyping and microarray analyses. The 3 primary cell populations, hcc-1, -2 and -3, and the 2 clones (clone-1/7 and -1/8) generated by limiting dilution from hcc-1 showed different expression prof

ORGANISM(S): Homo sapiens

SUBMITTER: Paolo Rebulla 

PROVIDER: E-GEOD-24482 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress