Expression of microRNAs and their gene targets are dysregulated in pre-invasive breast cancer (mRNA)

ABSTRACT: Introduction: microRNAs (miRNAs) are short non-coding RNAs that negatively regulate gene expression and may play a causal role in invasive breast cancer. Since many genetic aberrations of invasive disease are detectable in earlier stages, we hypothesized that miRNA expression dysregulation and the predicted changes in gene expression would also be found in early breast neoplasias. Methods: Expression profiling of 365 miRNAs by RT-qPCR was combined with laser-capture microdissection to obtain an epithelial specific miRNA expression signature of normal breast epithelium (n=9) and of paired samples of histologically normal epithelium (HN) and ductal carcinoma in situ (DCIS) (n=16). To determine how miRNAs may control the expression of co-dysregulated mRNAs we also performed gene expression

ORGANISM(S): Homo sapiens

SUBMITTER: Bethany Hannafon 

PROVIDER: E-GEOD-24506 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress