B-type lamins differentially associate with specific genes during differentiation without directly affecting their expression

ABSTRACT: B-type lamins, the major structural component of the nuclear lamina, is thought to play a repressive role in the expression of many of its bound genes whose silencing are known to be critical for cell differentiation during early development. Using global mapping of lamin-B-chromatin interaction, we find that the binding to lamin-B indeed correlated with gene silencing in mouse embryonic stem cells (ESC) and ESC-derived trophectoderm (TE) lineage cells. To address whether lamin-B directly suppresses the expression of the bound genes, we have derived ESCs from lamin-B-null mouse blastocysts. Unexpectedly, microarray analyses of ESCs and TE cells show that B-type lamins do not repress the expression of their bound genes. Furthermore, the knockout mice that do not express any B-type lamin

ORGANISM(S): Mus musculus

SUBMITTER: Minoru Ko 

PROVIDER: E-GEOD-24532 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress