Transcriptional Activation by Oct4 Is Sufficient for the Maintenance and Induction of Pluripotency

ABSTRACT: Oct4 is an essential regulator of embryonic stem (ES) cell pluripotency in vivo and in vitro, as well as a key mediator of the reprogramming of somatic cells to form induced pluriopotent stem (iPS) cells. It is not known whether activation and/or repression of specific genes by Oct4 is relevant to these functions. Here we show that fusion proteins containing the coding sequence of Oct4 or Xlpou91 (the Xenopus homologue of Oct4) fused to activating regions, but not those fused to repressing regions, behave as Oct4, suppressing differentiation and promoting maintenance of undifferentiated phenotypes in vivo and in vitro. An Oct4 activation domain fusion supported ES cell self-renewal in vitro at lower concentrations than required for Oct4 while alleviating the ordinary requirement for the cy

ORGANISM(S): Mus musculus

SUBMITTER: Minoru Ko 

PROVIDER: E-GEOD-24563 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress