A biophysical DNA binding model for the LEAFY transcription factor reveals the evolutionary fluidity of its binding sites

ABSTRACT: Despite great advances in sequencing capacity, generating functional information for non-model organisms remains a challenge. One solution lies in an improved ability to predict genetic circuits based on primary DNA sequence combined with the characterization of regulatory molecules from model species. Here, we focus on the LEAFY (LFY) transcription factor, a conserved master regulator of floral development. Starting with biochemical and structural information, we built a biophysical model describing LFY DNA binding specificity in vitro that accurately predicts in vivo LFY binding sites in the Arabidopsis thaliana genome. Extending the model to other species, we show that it can correctly identify functional homologs of known LFY targets from Arabidopsis thaliana in other angiosperms, even

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Felix Ott 

PROVIDER: E-GEOD-24568 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress