Metastatic and Nonmetastatic canine soft tissue sarcoma comparison

ABSTRACT: We hypothesize that there is a gene signature which will improve our ability to predict development of metastatic disease in STS patients. The objective of this study was to determine the feasibility of using cDNA microarray and quantitative real-time PCR (qRT-PCR) analysis to determine gene expression patterns in metastatic versus non-metastatic canine STSs, given the inherent heterogeneity of this group of tumors. Five STSs from dogs with metastatic disease were evaluated in comparison to eight STSs from dogs without metastasis. Tumor RNA was extracted, processed and labeled for application to the Affymetrix Canine Genechip 2.0 Array. Array fluorescence was normalized using D-Chip software and data analysis was performed with JMP/Genomics. Differential gene expression was validated

ORGANISM(S): Canis lupus familiaris

SUBMITTER: Marlene Hauck 

PROVIDER: E-GEOD-24601 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress