Direct conversion of human fibroblasts to multilineage blood progenitors

ABSTRACT: Similar to embryo-derived stem cells, application of human induced pluripotent stem cells (iPSCs) is limited by our understanding of lineage specification. Here, we demonstrate the ability to generate progenitors and mature cells of the hematopoietic fate directly from human dermal fibroblasts without establishing pluripotency. POU domain activation of hematopoietic transcription factors by ectopic expression of Oct-4, together with specific cytokine treatment, allowed generation of cells expressing the pan-leukocyte marker CD45. These unique fibroblast-derived cells gave rise to granulocytic, monocytic, megakaryocytic, and erythroid lineages, and demonstrated in vivo engraftment capacity. Distinct from PSC-derived hematopoietic cells, adult and not embryonic hematopoietic programs are act

ORGANISM(S): Homo sapiens

SUBMITTER: Ryan Mitchell 

PROVIDER: E-GEOD-24621 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress