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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from 1-N-naphthylphthalamic acid (NPA) treated stems of Arabidopsis thaliana

ABSTRACT: In-vivo induced establishment and activity of the interfascicular cambium in Arabidopsis thaliana stems under NPA treatments. We used microarrays to detail the global programme of gene expression underlying the establishment and activity of the interfascicular cambium. 3 mm stem pieces were collected after being in treated with NPA for a week in order to stop the polar auxin transport and induce secondary growth. Collected samples were used for RNA extraction and hybridization on Affymetrix microarrays. We aimed to obtain genes responsible for auxin-induced cambium establishment and activity.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Javier Agusti

PROVIDER: E-GEOD-24763 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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WOX4 imparts auxin responsiveness to cambium cells in Arabidopsis.

Suer Stefanie S Agusti Javier J Sanchez Pablo P Schwarz Martina M Greb Thomas T

The Plant cell 20110916 9

Multipotent stem cell populations, the meristems, are fundamental for the indeterminate growth of plant bodies. One of these meristems, the cambium, is responsible for extended root and stem thickening. Strikingly, although the pivotal role of the plant hormone auxin in promoting cambium activity has been known for decades, the molecular basis of auxin responsiveness on the level of cambium cells has so far been elusive. Here, we reveal that auxin-dependent cambium stimulation requires the homeo ...[more]

PMID: 21926336

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Expression data from in-vitro induced Interfascicular Cambium of Arabidopsis thaliana

Project description:In-vitro induced establishment and activity of the interfascicular cambium in Arabidopsis thaliana stems under auxin treatments. We used microarrays to detail the global programme of gene expression underlying the establishment and activity of the interfascicular cambium and identified tissue-speciffic up-regulated genes during this process. Different tissue types from in-vitro auxin treated stems were selected at successive stages of cambium establishment using laser capture microdissection for RNA extraction and hybridization on Affymetrix microarrays. We aimed to obtain genes exclussively upregulated in the interfascicular region responsible for cambium establishment and activity.

2010-12-31 | E-GEOD-22947 | biostudies-arrayexpress

Expression data from NPA treated stems of Arabidopsis thaliana.

Project description:In-vivo induced establishment and activity of the interfascicular cambium in Arabidopsis thaliana stems under NPA treatments. We used microarrays to detail the global programme of gene expression underlying the establishment and activity of the interfascicular cambium.

2011-09-01 | GSE24763 | GEO

Expression data from in-vitro induced Interfascicular Cambium of Arabidopsis thaliana

2010-12-31 | GSE22947 | GEO

Transcription profiling of Arabidopsis slr1 mutant and wild type lateral root segments treated with auxin

Project description:Lateral root initiation was used as a model system to study the mechanisms behind auxin-induced cell division. Genome-wide transcriptional changes were monitored during the early steps of lateral root initiation. Inclusion of the dominant auxin signaling mutant solitary root1 (slr1) identified genes involved in lateral root initiation that act downstream of the AUX/IAA signaling pathway. Interestingly, key components of the cell cycle machinery were strongly defective in slr1, suggesting a direct link between AUX/IAA signaling and core cell cycle regulation. However, induction of the cell cycle in the mutant background by overexpression of the D-type cyclin (CYCD3;1) was able to trigger complete rounds of cell division in the pericycle that did not result in lateral root formation. Therefore, lateral root initiation can only take place when cell cycle activation is accompanied by cell fate respecification of pericycle cells. The microarray data also yielded evidence for the existence of both negative and positive feedback mechanisms that regulate auxin homeostasis and signal transduction in the pericycle, thereby fine-tuning the process of lateral root initiation. Experiment Overall Design: Seedlings of both wild type (Col-0) and the lateral root defective mutant (slr-1) were germinated on MS medium supplemented with 10Î¼M NPA (=auxin transport inhibitor). Three days after germination, such seedlings were transferred to MS supplemented with 10Î¼M NAA for 0h, 2h and 6h respectively. The segment between root meristem and root-hypocotyl junction was harvested from about 1500 seedling per time point. All treatments were repeated biologically. 5.8 Î¼g total RNA was used for the preparation of biotinylated cRNA. Labeled RNA was hybridised to ATH1 Affymetrix chips. The resulting data was MAS5.0 normalised.

2007-07-13 | E-GEOD-3350 | biostudies-arrayexpress

Expression data from stem samples taken from the base and the first internode of Arabidopsis wildtype and wox4-1 plants

2011-09-30 | GSE24781 | GEO

Rice Lateral Root Inducible System timeseries

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2023-08-24 | E-MTAB-13094 | biostudies-arrayexpress

Influence of Nod Factors on early developmental stages of root development in Medicago truncatula

Project description:We would like to know how symbiotic molecules such as Nod Factors (NF) influence lateral root (LR) development in M. truncatula. We have preliminary evidence that this action is through early stages of root development. Auxin is the major phytohormone controlling LR development and we also have evidence that NF interfere with auxin for the control of LR development. This transcriptomic study aims at finding new molecular targets that would be responsive to auxin and NF treatment, even at a higher level by the combination of both auxin and NF. Such targets could be involved in the control or early development stages of LR that would be controlled both by auxin and NF treatment. - 2 day old M. truncatula (accession A17) plantlets grown on M medium were transferred for 2 days on M medium+ 10-5M NPA (1-N-Naphthylphthalamic acid, auxin transport inhibitor) then transferred for 10 h on M medium containing 10-6 M NAA (naphthalene-1-acetic acid, permeant auxin analog) or 10-7 M Nod factors (NF) or a combination of both (10-6 M NAA + 10-7 M NF). These are compared to mock treated (solvent only) plants. 12plex_med_2013_04 12 dye-swap - treated vs untreated comparison

2016-06-01 | E-GEOD-74099 | biostudies-arrayexpress

Transcriptome profiles and comparison of Arabidopsis thaliana woody (soc1-ful) and WT (Col-0) stems.

Project description:The douple mutant Arabidopsis thaliana soc1 ful, in contrast with WT, produces an interfascicular cambium and a large wood cylinder is the flowering stem. We present the RNAseq data for polyA mRNA of different developmental stages of cambium and wood formation in Arabidopsis thaliana. We sequenced 7 stages; 4 in the woody mutant soc1-6 ful-7 (herbaceous, cambium initiation, wood initiation and leaf) and 3 stages in the WT Col-0 (herbaceous, cambium and leaf). The corresponding stem anatomy is also presented in the manuscript indicating the stage of cambium development and the production of secondary xylem.

2016-05-21 | GSE69167 | GEO

NPA-induced vascular overgrowth in Arabidopsis

Project description:We used N-(1-naphthyl) phthalamic acid (NPA)-induced vascular overgrowth in Arabidopsis leaves to look for differential up-regulation of genes in NPA-treated tissues that may be involved in vascular differentiation. Arabidopsis thaliana Col-0 plants were grown for approximately 2 weeks on solid ATS medium (1) containing a final concentration of 10 um NPA (dissolved in DMSO) or an equivalent volume of DMSO (control). At this stage plants had approximately 6 rosette leaves. RNA was prepared from entire shoot tissues of control (DMSO) or NPA-treated plants.(1) Lincoln et al., 1990. Plant Cell 2: 1071-1080. 2 samples were used in this experiment.

2009-12-05 | E-GEOD-19262 | biostudies-arrayexpress

Expression data from stem samples taken from the base and the first internode of Arabidopsis wildtype and wox4-1 plants

Project description:Stem samples of wildtype Columbia plants and the wox4-1 mutant (Gabi_462G01) were analyzed in order to draw a connection between general transcriptomic changes during interfascicular formation in the wildtype and WOX4-dependent gene regulation during this process. We used microarrays to identify factors downstream of WOX4 in the formation of the interfascicular cambium. Transcriptional profiling, comparing wildtype Columbia and wox4-1 plants. RNA was extracted from 3 biological replicates per plant line and sample type (stem base, first internode), each consisting of a pool of 13-17 stem samples.

2011-09-29 | E-GEOD-24781 | biostudies-arrayexpress

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