Digital gene expression profiling for maize development - 2mm ear libraries, B73 vs. ramosa3 mutant

ABSTRACT: Genome-wide expression signatures detect specific perturbations in developmental programs and contribute to functional resolution of key regulatory networks. In maize (Zea mays) inflorescences, mutations in the RAMOSA (RA) genes affect determinacy of axillary meristems and thus alter branching patterns, an important agronomic trait. In this work, we developed and tested a framework for analysis of tag-based, digital gene expression (DGE) profiles using Illumina’s high-throughput sequencing technology and the newly-assembled B73 maize reference genome. We also used a mutation in the RA3 gene to identify putative expression signatures specific to stem cell fate in determinacy of axillary meristems. The RA3 gene encodes a trehalose-6-phosphate-phosphatase and may act at the interface betw

ORGANISM(S): Zea mays

SUBMITTER: Andrea Eveland 

PROVIDER: E-GEOD-24788 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress