Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

11 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Impact of miR-132 and miR-381 on endogenous forskolin responsive CREB targets in HEK293T cells

ABSTRACT: Expression profiling of HEK293T cells transfected with miR-neg (negative control), miR-132 or miR-381 mimics and treated with forskolin for 0, 1 or 4hrs. HEK293T cells were reverse transfected with the respective miRNA mimics. 72hrs post-transfection, cells were treated with forskolin for 0, 1 or 4hrs. Total RNA was then extracted. Expression profiling was carried out with Affymetrix arrays. Experiment was performed in duplicate. Total of 18 samples.

ORGANISM(S): Homo sapiens

SUBMITTER: Anthony Olarerin-George

PROVIDER: E-GEOD-24812 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Impact of miR-132 and miR-381 on endogenous forskolin responsive CREB targets in HEK293T cells

Project description:Expression profiling of HEK293T cells transfected with miR-neg (negative control), miR-132 or miR-381 mimics and treated with forskolin for 0, 1 or 4hrs.

2011-07-01 | GSE24812 | GEO

Genes regulated by miR-328-3p in ovarian cancer cells

Project description:To identify genes regulated by miR-328-3p, we transfected miR-328-3p mimics in ovarian cancer cell line OV2008, and compared the gene expression profiles between miR-328-3p mimics transfected and Negative Control miRNA-transfected cells.

2019-01-01 | GSE119297 | GEO

Transcriptome wide target profiling for miR-199a-5p and miR-424-3p.

Project description:Whole transcriptome Identification of direct targets of miR-199a-5p and miR-424-3p using biotinylated pull-downs found that both miRNAs are likely to have a role in the cell cycle. HEK293T cells were transfected with biotinylated miRNAs (either miR-199a-5p or miR-424-3p). The miRNAs and target mRNA were pulled down with streptavidin and compared to the input control.

2013-12-02 | E-GEOD-40406 | biostudies-arrayexpress

Negative control and mir-342-3p mimics-transfected RAW264.7 mouse macrophages.

Project description:RAW264.7 mouse macrophages were transfected with negative control and miR-342-3p mimics and subjected to microarray analysis 18 hours after the transfection. We used microarray to obtain global mRNA expression data of negative control and miR-342-3p mimics-transfected RAW264.7 cells.

2016-04-29 | GSE71642 | GEO

Homo sapiens

Project description:Impact of miR-132 and miR-381 on endogenous forskolin responsive CREB targets in HEK293T cells

| PRJNA132309 | ENA

Genome-wide analysis of miR-509-3p-mediated gene silencing in A549 lung cancer cells

Project description:Analysis of changes in gene expression after transfection with miR-509-3p mimic in A549 cells Total RNA was obtained from A549 cells transfected with miR-509-3p and negative control mimics, and gene expression was compared using microarrays.

2014-05-31 | E-GEOD-47522 | biostudies-arrayexpress

Differential expression of genes after over expression of hsa-miR-128 in human embryonic kidney cells (HEK293T cells)

Project description:The experiment was deigned to identify the genes which get altered after the over expression of hsa-miR-128 in HEK293T cells. The HEK293T cells plated in 6-well plate were transfected with 4 micrograms of cloned miR-128 (p128) in three biological replicates. Biological triplicates of 2 samples were used viz. control HEK293T cells (c), HEK293T cells transfected with 4 micrograms of the cloned hsa-miR-128(t).

2012-05-09 | E-GEOD-31297 | biostudies-arrayexpress

Real-time quantitative PCR analysis of miRNA in mouse peritoneal macrophages upon miR-33 or miR-33* modulation

Project description:Mouse peritoneal macrophages were transfected with 80-120 nM miRIDIAN miRNA mimics (miR-mimic-33/miR-mimic-33*) or with 80-120 nM miRIDIAN miRNA inhibitors (anti-miR-33 ASO/anti-miR-33*ASO) Control samples were treated with an equal concentration of a non-targeting control mimics sequence (control mimic) or inhibitor negative control sequence (control aso), to control for non-specific effects in miRNA experiments.

2016-04-18 | GSE79055 | GEO

miRNA expression in CTRL1790 cells transfected with miR mimics

Project description:800-plex Nanostring miRNA experession from CRL1790 cells transfected with miR mimics or no-transfection control.

2020-08-01 | GSE133586 | GEO

RNA-Seq of mouse microglia under LPS stimulation and miR-106 overexpression

Project description:We investigated the expression patterns of transcripts in mouse primary microglia that are stimulated by LPS and transfected with miR-106b mimics. Three experiment groups were utilized in the RNA-seq, that are PBS+mimics control group, LPS+mimics group, and LPS+miR-106b mimics group. Samples were collected 2 days after LPS treatment and mimics transfection.

2021-10-31 | E-MTAB-10946 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data