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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional changes in Sohlh1 knockout and Sohlh2 knockout mouse newborn ovaries

ABSTRACT: Sohlh1 and Sohlh2 are germ cell-specific basic helix-loop-helix transcription factors critical in early folliculogenesis. We discovered that Sohlh1 and Sohlh2 knockout females lose oocytes after birth and few remains by postnatal day 14. Here, we show that many genes preferentially expressed in the oocytes are misregulated by Sohlh1 and/or Sohlh2 deficiency. Total RNA samples isolated from wildtype, Sohlh1 KO and Sohlh2 KO mouse newborn ovaries were arrayed on the Illumina BeadChip MouseWG-6 2.0 arrays. We have 4 replicates for wildtype and Sohlh1 KO and 3 replicates for Sohlh2 KO.

ORGANISM(S): Mus musculus

SUBMITTER: Hyo Ahn

PROVIDER: E-GEOD-24816 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional changes in Sohlh1/Sohlh2 double knockout newborn mouse ovaries

Project description:Sohlh1 and Sohlh2 are germ cell-specific basic helix-loop-helix transcription factors critical in early folliculogenesis. Differential genes expression by both Sohlh1 and Sohlh2 deficiency in mouse newborn ovaries was accessed using microarray. RNA samples from Sohlh1/ Sohlh2 double knockout and wild-type newborn ovaries were arrayed on the Illumina beadchip mouse WG-6 2.0. Total RNA isolated from wildtype and Sohlh1/Sohlh2 double KO mouse newborn ovary were used to run Illumina BeadChip MouseWG-6 2.0 arrays.

2010-10-22 | E-GEOD-24815 | biostudies-arrayexpress

Transcriptional changes in Sohlh1 knockout and Sohlh2 knockout mouse newborn ovaries

Project description:Sohlh1 and Sohlh2 are germ cell-specific basic helix-loop-helix transcription factors critical in early folliculogenesis. We discovered that Sohlh1 and Sohlh2 knockout females lose oocytes after birth and few remains by postnatal day 14. Here, we show that many genes preferentially expressed in the oocytes are misregulated by Sohlh1 and/or Sohlh2 deficiency.

2010-10-22 | GSE24816 | GEO

Transcriptional changes in Sohlh1/Sohlh2 double knockout newborn mouse ovaries

2010-10-22 | GSE24815 | GEO

Regulation of early folliculogenesis by Sohlh1 and Sohlh2 in mouse ovary

Project description:This SuperSeries is composed of the following subset Series: GSE24815: Transcriptional changes in Sohlh1/Sohlh2 double knockout mouse newborn ovaries GSE24816: Transcriptional changes in Sohlh1 knockout and Sohlh2 knockout mouse newborn ovaries Refer to individual Series

2010-10-22 | E-GEOD-24817 | biostudies-arrayexpress

Expression data from testis of day-7 Sohlh1 knock-out mice

Project description:Sohlh1 and Sohlh2 encode a germ cell-specific basic helix-loop-helix transcriptional regulator critical in spermatogonial differentiation. Seven-day-old Sohlh1 or Sohlh2 knockout and wild-type testes were arrayed on the Affy 430 2.0 platform.

2010-04-27 | GSE21525 | GEO

Expression data from testis of day-7 Sohlh1 knock-out mice

2010-05-07 | E-GEOD-21525 | biostudies-arrayexpress

FIGLA, LHX8 and SOHLH1 transcription factor networks regulate mouse oocyte growth and differentiation

Project description:Germ-cell transcription factors control gene networks that regulate primordial follicle formation and oocyte differentiation during early, postnatal mouse oogenesis. Taking advantage of gene-edited mice lacking transcription factors expressed in female germ cells, we analyzed global gene expression profiles in perinatal ovaries from wildtype, FiglaNull, Lhx8Null and SohlhNull mice. Figla deficiency dysregulates expression of meiosis-related genes (e.g., Sycp3, Rad51 and Msy2) and a variety of genes (e.g., Nobox, Lhx8, Taf4b, Sohlh1, Sohlh2 and Gdf9) associated with oocyte growth and differentiation. The absence of FIGLA significantly impedes meiotic progression, causes DNA damage and results in oocyte apoptosis. Moreover, we find that FIGLA and other transcriptional regulators (e.g., NOBOX, LHX8, SOHLH1 and SOHLH2) are co-expressed in the same subset of germ cells in perinatal ovaries and Figla ablation dramatically disrupts KIT, NOBOX, LHX8, SOHLH1 and SOHLH2 expression. In addition, not only do FIGLA, SOHLH1 and LHX8 cross-regulate each other, they also cooperate by direct interaction with each during early oocyte development and share downstream gene targets. Thus, our findings substantiate a major role for FIGLA, LHX8 and SOHLH1 as multifunctional regulators of networks necessary for oocyte maintenance and differentiation during early folliculogenesis.

2019-11-06 | GSE139966 | GEO

Project description:Gene expression between wild-type, Sohlh1 KO, Sohlh2 ko and Sohlh1/2 DKO

| PRJNA575139 | ENA

Project description:Regulation of early folliculogenesis by Sohlh1 and Sohlh2 in mouse ovary

| PRJNA132195 | ENA

FIGLA, an oocyte-specific transcription factor, affects ovarian gene expression

Project description:Ovarian folliculogenesis in mammals is a complex process involving cross talk between germ and somatic cells. Carefully orchestrated expression of transcription factors, cell adhesion molecules and growth factors are required for success. We have identified a germ-cell specific basic helix-loop-helix transcription factor, FIGLA (Factor In GermLine, Alpha) and demonstrated its involvement in two independent developmental processes: formation of primordial follicle and coordinate expression of zona pellucida genes. Taking advantage of Figla null mouse line, we have used microarrays to identify potential downstream target genes. Using high stringent cut offs, we find that FIGLA functions a key regulatory molecule in coordinating expression of the NALP family of genes, genes of known oocyte-specific expression and a set of functionally un-annotated genes. These data implicate FIGLA as a central regulator of oocyte-specific genes that play role in folliculogenesis and early development. We analyzed 8 groups of arrays, 26 arrays total. Four arrays of WT newborn ovary RNA (cy5) versus KO newborn ovary RNA (Cy3), then four arrays of the same samples dye-reversed. Three arrays of WT E17.5 ovary RNA (cy5) versus KO E17.5 ovary RNA (Cy3), then three arrays of the same samples dye-reversed. Three arrays of WT E14.5 ovary RNA (cy5) versus KO E14.5 ovary RNA (Cy3), then three arrays of the same samples dye-reversed. Three arrays of WT E12.5 ovary RNA (cy5) versus KO E12.5 ovary RNA (Cy3), then three arrays of the same samples dye-reversed.

2007-08-17 | E-GEOD-5558 | biostudies-arrayexpress

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