A wholly-defined Agilent microarray spike-in dataset

ABSTRACT: We have generated a wholly defined spike-in dataset for Agilent microarrays consisting of 12 arrays with more than 2000 differentially expressed, and approximately 3600 background, cRNAs. The composition of this “Ag Spike”dataset is identical to that of our previous Platinum Spike dataset (GSE21344) and therefore allows direct cross-platform comparison. Comparison between the Ag Spike and Platinum Spike studies shows high agreement between results obtained using the Affymetrix and Agilent platforms. We used the same 28 PCR pools that had been used before to generate the Platinum-Spike dataset (GSE21344). These pools contain PCR products from 5725 Drosophila Gene Collection release 1.0 (DGCr1) cDNA clones. We mixed PCR products from all pools together with specified relative abundance t

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Qianqian Zhu 

PROVIDER: E-GEOD-24866 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress