Acute high dose X-irradiation-induced genomic changes in a lung tumor cell line

ABSTRACT: Accidents with ionizing radiation (IR) often involve acute high dose exposures that can lead to acute radiation syndrome and late effects. IR can induce genomic lesions, cell death or carcinogenesis. Here, we investigated acute IR-induced cellular genomic signatures at the genome wide level. After exposing the adenocarcinoma cell line A549 to an acute 6 Gy 240 kV X-Ray dose, four surviving clonogenic cells were recovered by minimal dilution and further expanded and analyzed by cytogenetics, chromosome painting and tiling-path array CGH, with the non-irradiated clone0 serving as control. It was found that acute X-ray exposure induced changes in modal chromosome number and specific translocations in the four irradiation surviving clones. Furthermore, clone4 displayed an increased radiosensi

ORGANISM(S): Homo sapiens

SUBMITTER: Artur Muradyan 

PROVIDER: E-GEOD-24876 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress