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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression in response to genetic and chemical perturbations of chromatin structure

ABSTRACT: Microarray expression profiling was used to identify genes expressed misexpressed in wild-type Arabidopsis seedlings treated with 5-aza-2’ deoxyctidine (5AC) or trichostatin A (TSA), and in decrease in dna methylation1 (ddm1) mutant seedlings. 10 day-old Arabidopsis seedlings (ecotype Ws) were grown in 0.5X MS media (containing 1% sucrose) at 21 C in continuous white light. Media (including 5AC and TSA) were refreshed after 5 days of growth. RNA was extracted using the Qiagen Plant RNeasy kit. Three biological replicates for each treatment and genotype were used.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Karen Hudson

PROVIDER: E-GEOD-25067 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Changes in global gene expression in response to chemical and genetic perturbation of chromatin structure.

Hudson Karen K Luo Song S Hagemann Nicole N Preuss Daphne D

PloS one 20110603 6

DNA methylation is important for controlling gene expression in all eukaryotes. Microarray analysis of mutant and chemically-treated Arabidopsis thaliana seedlings with reduced DNA methylation revealed an altered gene expression profile after treatment with the DNA methylation inhibitor 5-aza-2' deoxycytidine (5-AC), which included the upregulation of expression of many transposable elements. DNA damage-response genes were also coordinately upregulated by 5-AC treatment. In the ddm1 mutant, more ...[more]

PMID: 21673996

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Gene expression in response to genetic and chemical perturbations of chromatin structure

Project description:Microarray expression profiling was used to identify genes expressed misexpressed in wild-type Arabidopsis seedlings treated with 5-aza-2’ deoxyctidine (5AC) or trichostatin A (TSA), and in decrease in dna methylation1 (ddm1) mutant seedlings.

2011-06-03 | GSE25067 | GEO

Interactions between reactive oxygen species stress and brassinosteroid hormone signaling in Arabidopsis seedlings

Project description:We were interested in investigating the transcriptome responses to exogenous applications of brassinosteroid hormone when Arabidopsis seedlings are pre-stressed with a reactive oxygen species, hydrogen peroxide. We were interested in seeing which subsets of BR-responsive gene transcripts were most affected and how BR-responsive gene transcripts responded to increasing concentrations of hydrogen peroxide both as a whole and individually. Liquid culture Arabidopsis seedlings are grown under standard conditions. Hydrogen peroxide is added at various concentrations to pre-stress the seedlings. Following this pretreatment, the seedlings are then treated with brassinosteroid (BR) hormone (epi-brassinolide, BL). Following this treatment, seedlings are harvested and total RNA is extracted for genome-wide transcriptome analysis.

2011-02-28 | E-GEOD-20756 | biostudies-arrayexpress

Transcriptional profiles between mp mutant seedlings and transgenics carrying the dexamethasone-inducible GR-bdl protein

Project description:In order to identify targets of the transcription factor AUXIN RESPONSE FACTOR5 / MONOPTEROS (ARF5/MP), we compared transcriptomes of mp-B4149 mutant seedlings (9 day-old) and seedlings carrying the dexamethasone-inducible version of the MP inhibitor protein BODENLOS (GR-bdl). Without dexamethasone (DEX) treatment, this line is identical to the wild-type, while DEX treatment leads to strong inhibition of ARF-dependent transcription. To remove all endogenous MP-inhibiting Aux/IAA proteins, we treated mp or GR-bdl seedlings during 1 hour with auxin (50 micromolar Indole-3-Acetic Acid), either with or without a pretreatment with 10 micromolar DEX for 1 hour. Genes that are activated by MP are expected to br downregulated in mp seedlings and in the GR-bdl line afer DEX treatment. We used biological duplicates for each of the three treatments. Keywords: Transcriptional profiling of transcription factor targets Mp mutant and GR-bdl seeds were germinated on solid 1/2MS media, and transferred to liquid medium after 9 days of horizontal growth. Duplicate cultures were generated for mp mutants, and quadruplicates for the GR-bdl line. 10 micromolar DEX was added to both mp cultures and to two of the GR-bdl cultures, and seedlings were gently shaken during 1 hour. Then, 50 micromolar IAA was added to all 6 cultures and seedlings were incubated with gentle shaking for another hour. Total RNA was isolated from the seedlings using a QIAgen RNeasy kit, and reverse transcription, in-vitro transcription, cRNA labling and fragemntation was done according to established methods (Schmid et al., Nature Genetics [2005] 37, 501-506). Labeled cRNA was hybridized to Arabidopsis ATH1 arrays.

2010-04-12 | E-GEOD-13881 | biostudies-arrayexpress

Auxin stimulates brassinosteroid biosynthesis in Arabidopsis roots

Project description:We found that auxin stimulates gene expression of DWF4, which encodes a rate-dertermining step in brassinosteroid biosynthesis pathways. This increased gene expressioin subsequently led to elevation of the biosynthetic flux in Arabidopsis roots. To determine the list of genes that are regulated by auxin-synthesizing brassinosteroids, we challenged Arabidopsis seedlings with either auxin only or auxin plus brassinosteroid biosynthetic inhibitor brassinazole. Keywords: Hormone treatment Arabidopsis seedlings (Columbia ecotype) were grown for 10 d on 1× MS agar-solidified media under long-day conditions (16:8, white light and dark cycle). The seedlings were then transferred to 2 different liquid media containing either 10–7 M 2,4-D or 10–7 M 2,4-D plus 10–6 M brassinazole. After 8 h of treatment, the seedlings were blotted with paper towels to remove excess media and subject to total RNA isolation. Total RNAs isolated from each batch were prepared from 3 replicate seedlings using an RNeasy plant mini kit (Qiagen, Germany).

2011-02-15 | E-GEOD-12964 | biostudies-arrayexpress

RNA-seq of Arabidopsis Col-0, snrk2.4, double snrk2.4/2.10 and quintuple snrk2.1/2.4/2.5/2.9/2.10 mutants from control (0mM) and salt stress (150mM NaCl for 1hour) treatment.

Project description:The purpose of this experiment was to investigate the downstream evens of Arabidopsis subclass 1 SnRK2 protein kinases signaling pathway. Seedlings were grown in 100 ml liquid media containing 0.5xMurashi-Skoog basal salt, 0.5% sucrose, 1% M.E.S. monohydrate, pH 5.8 (KOH) with shaking (120 rpm). Ten days-old seedlings were treated with 150 mM NaCl in 0.1xMS media (salt stress) or 0.1xMS media (control) for 1hour.

2019-07-11 | E-MTAB-8073 | biostudies-arrayexpress

The effect of sucrose and sulfamethoxazole on the Arabidopsis transcriptome

Project description:A chemical screen was performed in search of compounds that modify plant responses to sucrose. This screen uncovered that sulfamethoxazole (SMX), a folate biosynthesis inhibitor, acted synergistically with sucrose to inhibit hypocotyl elongation, suggesting interaction between these two pathways. Transcriptome analysis was performed to identify changes in transcript abundance that may underpin crosstalk between sucrose and SMX. Three-day-old dark-grown seedlings were treated to sucrose and SMX at concentrations that induced no change in hypocotyl elongation when administered independently, yet restricted elongation when both were present in the growth media (10mM and 0.2µM, respectively). This analysis uncovered multiple core auxin signalling components that exhibit altered transcript abundance in response to co-treatment with sucrose and SMX, suggesting that auxin signalling mediates crosstalk between these two pathways. This study highlights an input through which metabolic status can shape plant growth and development through hormone signalling. 12 arrays total. Three arrays as non-treated control, three arrays from seedlings raised in presence of 0.2µM SMX, three arrays from seedlings raised in presence of 10mM sucrose, and three arrays from seeldings raised in presence of both 10mM sucrose and 0.2µM SMX. Three biological replicates were produced for each growth treatment.

2013-06-30 | E-GEOD-37484 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type, 35S-ARR7, arr7 and 35S-ARR15 treated with cytokinin, auxin or both

Project description:Above ground tissue of 10 day old Arabidopsis seedlings of Col wild-type, 35S-ARR7, arr7, 35S-ARR15 was treated with Cytokinin (benzyladenine), Auxin (indole-3-acetic acid) or both.

2010-12-31 | E-MEXP-2270 | biostudies-arrayexpress

Expression analysis of Arabidopsis seedlings 3h and 2h after transfer to sucrose

Project description:Seedlings are grown on a mesh covering MS media without carbon source, and subsequently transferred at 9 DAS to control media without sucrose or medium supplemented with 15 mM sucrose. 3 hours and 24 hours after transfer, seedlings were harvested and the third leaf micro-dissected for RNA extraction.

2017-12-14 | E-MTAB-4262 | biostudies-arrayexpress

Transcription profiling of Arabidopsis seedlings treated with NAE(12:0)

Project description:Transcript profiling and gene expression studies in NAE-treated seedlings: Seeds were germinated and seedlings maintained for 4 d in liquid MS media supplemented with 35 uM NAE(12:0)(N-lauroylethanolamine) prior to RNA isolation.

2007-11-03 | E-MEXP-1310 | biostudies-arrayexpress

Transcription profiling of Arabidopsis after deuterium oxide treatment

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2009-09-25 | E-GEOD-18153 | biostudies-arrayexpress

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