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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression in response to progestin R5020

ABSTRACT: Breast cancer cell lines containing the progesterone receptor respond to progestins, altering expression of a subset of genes. In a previously published experiment of inducible knock-down of histone H1 isoforms with an shRNA-expression lentivector (GSE12299), we modified the breast cancer cell line T47D with different vectors. Here, we explored response to progestin R5020 of control cells generated with the empty shRNA-expression vector. Stable breast cancer-derived cell lines containing the empty vector for inducible shRNA expression were grown for two days in the absence of serum. Progestin R5020 or vehicle was added for 6 hours, in duplicate, and RNA was extracted for microarray hybridization.

ORGANISM(S): Homo sapiens

SUBMITTER: Lauro Sumoy

PROVIDER: E-GEOD-25077 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Progestins activate 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) in breast cancer cells.

Novellasdemunt Laura L Obach Mercè M Millán-Ariño Lluís L Manzano Anna A Ventura Francesc F Rosa Jose Luis JL Jordan Albert A Navarro-Sabate Aurea A Bartrons Ramon R

The Biochemical journal 20120301 2

PFKFB (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase) catalyses the synthesis and degradation of Fru-2,6-P2 (fructose-2,6-bisphosphate), a key modulator of glycolysis and gluconeogenesis. The PFKFB3 gene is extensively involved in cell proliferation owing to its key role in carbohydrate metabolism. In the present study we analyse its mechanism of regulation by progestins in breast cancer cells. We report that exposure of T47D cells to synthetic progestins (ORG2058 or norgestrel) leads to ...[more]

PMID: 22115192

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RNA-seq in two ER+ breast cancer cell lines with and without progestins

Project description:Exploring effect of progesterone/progestin treatment on gene expression Two cell lines, three conditions (Full Media with E2, E2+ Progesterone, Full Media + R5020 Progestin)

2015-05-22 | E-GEOD-68358 | biostudies-arrayexpress

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2015-05-15 | E-GEOD-67561 | biostudies-arrayexpress

ChIP-seq of ER, PR, p300 in two ER+ breast cancer cell lines treated with estrogen and with and without progestins

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2015-05-22 | E-GEOD-68355 | biostudies-arrayexpress

Homo sapiens

Project description:Gene expression in response to progestin R5020

| PRJNA134745 | ENA

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PrPC-dependent gene expression and cell signalling signature in Neuro2a cells. Role on mitogenic stimulation and cell morphology.

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2015-09-04 | E-GEOD-72708 | biostudies-arrayexpress

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