Transcriptome of an intestine-adapted Escherichia coli K-12 mutant selected by animal passage for superior colonization

ABSTRACT: Transcription profiling of wild type E. coli MG1655, intestine-adapted E. coli MG1655star, and E. coli MG1655 flhD mutant grown on glucose, mannose, and mucus. We previously isolated a spontaneous mutant of E. coli K-12, strain MG1655, following passage through the streptomycin-treated mouse intestine, which has colonization traits superior to the wild-type parent strain (Leatham, et. al., 2005, Infect Immun 73:8039-49) The intestine-adapted strain (E. coli MG1655star) grew faster on several different carbon sources compared to the wild-type and was non-motile due to deletion of the flhD gene. To further characterize E. coli MG1655star, we used several high-throughput genomic approaches. Whole-genome pyrosequencing did not reveal any changes on its genome, aside from the deletion at the fl

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Tyrrell Conway 

PROVIDER: E-GEOD-25106 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress