Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Expression data from dark-grown BR double mutant bzr1-1D;bri1-116, single mutant bri1-116, and wildtype


ABSTRACT: Molecular genetic analyses support a central role of BZR1 in Brassinosteroid (BR) regulation of plant development. The dominant bzr1-1D mutation, which stabilizes the BZR1 protein, completely suppresses the de-etiolated phenotype of the null bri1-116 mutant grown in the dark. Using microarray analysis, we identified genes differentially expressed in bri1-116 compared to wild type and genes differentially expressed in the bzr1-1D;bri1-116 double mutant compared to the bri1-116 single mutant. Consistent with the phenotypic suppression of bri1-116 by bzr1-1D, about 80% of the genes affected in bri1-116 were affected oppositely by bzr1-1D BZR1 regulated genes were generated from comparing genes differentially expressed by bzr1-1D;bri1-116 and bri1-116. Genes affected by BRI1 were generated from comparing differentially expressed genes of bri1-116 and Col control. ANOVA was used to find genes whose expression was different between bzr1-1D;bri1-116 and bri1-116 or between bri1-116 and Col samples [see Supplementary file below].

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Yu Sun 

PROVIDER: E-GEOD-25134 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Integration of brassinosteroid signal transduction with the transcription network for plant growth regulation in Arabidopsis.

Sun Yu Y   Fan Xi-Ying XY   Cao Dong-Mei DM   Tang Wenqiang W   He Kun K   Zhu Jia-Ying JY   He Jun-Xian JX   Bai Ming-Yi MY   Zhu Shengwei S   Oh Eunkyoo E   Patil Sunita S   Kim Tae-Wuk TW   Ji Hongkai H   Wong Wing Hong WH   Rhee Seung Y SY   Wang Zhi-Yong ZY  

Developmental cell 20101101 5


Brassinosteroids (BRs) regulate a wide range of developmental and physiological processes in plants through a receptor-kinase signaling pathway that controls the BZR transcription factors. Here, we use transcript profiling and chromatin-immunoprecipitation microarray (ChIP-chip) experiments to identify 953 BR-regulated BZR1 target (BRBT) genes. Functional studies of selected BRBTs further demonstrate roles in BR promotion of cell elongation. The BRBT genes reveal numerous molecular links between  ...[more]

Similar Datasets

2010-12-31 | GSE25134 | GEO
2011-11-09 | E-GEOD-25396 | biostudies-arrayexpress
2015-04-30 | E-GEOD-52966 | biostudies-arrayexpress
2011-11-09 | GSE25396 | GEO
| PRJNA142765 | ENA
2015-07-14 | E-GEOD-70843 | biostudies-arrayexpress
2015-07-14 | GSE70843 | GEO
2024-01-19 | GSE233415 | GEO
2022-12-15 | GSE181871 | GEO
2013-03-12 | E-GEOD-45009 | biostudies-arrayexpress