Differential gene expression in notochord and nerve-cord fate segregation in the Ciona intestinalis embryo

ABSTRACT: Fertilized eggs are plulipotent and during early embryogenesis, embryonic cells gradually restrict their developmental potential and are eventually destined to give rise to one type of cells. Molecular mechanism underlying developmental fate restriction is one of the major research subjects of developmental biology. Here we address this question by combining a classical technique of blastomere isolation with a modern technique of microarray analysis. During the 6th cleavage of the Ciona intestinalis embryo, from the 32-cell to the 64-cell stage, four mother cells divide into daughter cells with two distinct fates, one giving rise to notochord and the other to nerve-cord precursor cells. Nearly 500 notochord or nerve-cord precursor cells each were isolated, and difference in the quality of

ORGANISM(S): Ciona intestinalis

SUBMITTER: Lixy Yamada 

PROVIDER: E-GEOD-25184 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress