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Rap1a GTPase mVSM Knockout vs. Rescue mRNA Expression

ABSTRACT: Transcriptional profiling of mouse tail artery explanted microvascular smooth muscle (mVSM) cells comparing Rap1a -/- mVSM cells transduced with vector backbone (control) with Rap1a -/- mVSM cells transduced with a Rap1 CA 'rescue' plasmid. The experiment will compare mRNA expression in Rap1A -/- mVSMs and 'rescued'with Rap1A CA. Two-condition experiment, mVSM Rap1a -/- (vector control) vs. mVSM-RapCA. Biological replicates: 4 control replicates, 4 transduced replicates.

ORGANISM(S): Mus musculus

SUBMITTER: tushar garud

PROVIDER: E-GEOD-25190 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rap1a GTPase mVSM Knockout vs. Rescue mRNA Expression

Project description:Transcriptional profiling of mouse tail artery explanted microvascular smooth muscle (mVSM) cells comparing Rap1a -/- mVSM cells transduced with vector backbone (control) with Rap1a -/- mVSM cells transduced with a Rap1 CA 'rescue' plasmid. The experiment will compare mRNA expression in Rap1A -/- mVSMs and 'rescued'with Rap1A CA. Two-condition experiment, mVSM Rap1a -/- (vector control) vs. mVSM-RapCA. Biological replicates: 4 control replicates, 4 transduced replicates.

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