GC-rich Sequence Elements Recruit Polycomb Repressive Complex 2 (PRC2) in ES cells

ABSTRACT: Polycomb proteins are epigenetic regulators that localize to developmental loci in the early embryo where they mediate lineage-specific gene repression. In Drosophila, these repressors are recruited to sequence elements by DNA binding proteins associated with Polycomb repressive complex 2 (PRC2). However, the sequences that recruit PRC2 in mammalian cells have remained obscure. To address this, we integrated a series of engineered bacterial artificial chromosomes into embryonic stem (ES) cells and examined their chromatin. We found that a 44 kb region corresponding to the Zfpm2 locus initiates de novo recruitment of PRC2. We then pinpointed a CpG island within this locus as both necessary and sufficient for PRC2 recruitment. Based on this causal demonstration and prior genomic analyses, we

ORGANISM(S): Mus musculus

SUBMITTER: Bradley Bernstein 

PROVIDER: E-GEOD-25197 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress