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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Nucleosome mapping data from GR and cell-cycle regulated genes in human cell lines

ABSTRACT: Human UL3 cells (U20S derivatives containing GR and an MMTV-luc transgene) were treated with dexamethasone for 0, 1 or 4 hrs. Chromatin was harvested, digested with to ~70% mononucleosomes with MNase, and isolated mononucleosome fragments were used to probe custom Nimblegen genomic tiling microarrays. In addition, human HL60 cells were treated with or without DMSO to induce granulocyte differentiaton, and mononucleosome positions mapped. MNase digested bare DNA fragments of ~500 bp average length from UL3 cells was used as a control. Mononucleosome positions at tiled genes for UL3 cells +/- dexamethasone and HL60 cells +/- DMSO.

ORGANISM(S): Homo sapiens

SUBMITTER: Gavin Schnitzler

PROVIDER: E-GEOD-25281 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Multiple distinct stimuli increase measured nucleosome occupancy around human promoters.

Pham Chuong D CD Sims Hillel I HI Archer Trevor K TK Schnitzler Gavin R GR

PloS one 20110811 8

Nucleosomes can block access to transcription factors. Thus the precise localization of nucleosomes relative to transcription start sites and other factor binding sites is expected to be a critical component of transcriptional regulation. Recently developed microarray approaches have allowed the rapid mapping of nucleosome positions over hundreds of kilobases (kb) of human genomic DNA, although these approaches have not yet been widely used to measure chromatin changes associated with changes in ...[more]

PMID: 21853138

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