Kc_H2Av_Knockdown_600_mM_Salt_Extracted_Chromatin_Pellet

ABSTRACT: modENCODE_submission_2760 This submission comes from a modENCODE project of Steven Henikoff. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We applied genome-wide profiling to successive salt-extracted fractions of micrococcal nuclease-treated Drosophila chromatin. Chromatin fractions extracted with 80 mM or 150 mM NaCl after digestion contain predominantly mononucleosomes and represent classical "active" chromatin. Profiles of these low-salt soluble fractions display phased nucleosomes over transcriptionally active genes that are locally depleted of histone H3.3 and correspond closely to profiles of histone H2Av (H2A.Z) and RNA polymerase II. This correspondence suggests that transcription can result in loss of H3.3+H2Av nucleosomes and ge

ORGANISM(S): Drosophila melanogaster

SUBMITTER: DCC modENCODE 

PROVIDER: E-GEOD-25389 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress