Temporal response of DCs to LPS stimulation: 4sU_sequencing

ABSTRACT: Regulation of RNA levels is critical for the response to external stimuli and determined through the interplay between RNA production, processing and degradation. Despite the centrality of these processes, most global studies of RNA regulation do not distinguish their separate contributions and relatively little is known about how they are temporally integrated. Here, we combine metabolic labeling of RNA with advanced RNA quantification assays and computational modeling to estimate RNA transcription and degradation during the response of immune dendritic cells (DCs) to pathogens, a critical and tightly regulated step in innate immunity. We find that transcription regulation plays a major role in shaping most temporal changes in RNA levels, but that changes in degradation rate are important

ORGANISM(S): Mus musculus

SUBMITTER: Michal Rabani 

PROVIDER: E-GEOD-25432 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress