Project description:Cycloheximide treatment of cotton ovules Keywords: Antibiotic Treatment Single timepoint, 6 slides, 3 Biological replicates, each biological replicate having 2 technical replicates. Dyes were swapped between technical replicates.
Project description:Genomic approaches to the discovery of promoters for sustained expression in cotton (Gossypium hirsutum L.) under field conditions: expression analysis in transgenic cotton and Arabidopsis of a Rubisco small subunit promoter identified using EST sequence analysis and cDNA microarrays. RNAs from 8-week old plants were compared to RNAs from 10-, 14-, 20- and 22-week old plants on separate arrays each with a dye swap. The 20 and 22 weeks hybridisations each had a biological replicate (each with a dye swap) as well as a second independent hybridisation (with dye swap) with one of the 22 week RNAs. One of the 22-week slides was of low quality and had to be discarded.
Project description:GLB1, a class 1 haemoglobin gene from Arabidopsis thaliana (GLB1) is essential for plant survival following hypoxic stress. Keywords = haemoglobin, hypoxic stress, stress