EQUIVALENCE OF CONVENTIONALLY-DERIVED AND PARTHENOTE-DERIVED HUMAN EMBRYONIC STEM CELLS

ABSTRACT: Parthenote lines proliferated more slowly than conventional hESC lines, and yielded lower quantities of less mature differentiated cells in a neural progenitor cell (NPC) differentiation protocol. However, the cell lines performed similarly in a RPE differentiation protocol. The DNA methylation analysis showed similar general profiles, but the two cell types differed in methylation of imprinted genes. There were no major differences in gene expression between the lines before differentiation, but when differentiated into NPCs, the two cell types differed in expression of extracellular matrix (ECM) genes. Conclusions/Significance: These data show that hESC and phESC are similar in the undifferentiated state, and both cell types are capable of differentiation along neural lineages. The diff

ORGANISM(S): Homo sapiens

SUBMITTER: Julie Harness 

PROVIDER: E-GEOD-25538 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress