Transcription profiling of mouse embryonic autopods to identify differentially expressed genes in developing forlimb and hindlimb tissues

ABSTRACT: Transcriptomes of mouse embryonic autopods were generated detecting expression of approximately 26179 transcripts in the developing forelimb or hindlimb autopods, representing about 58 % of the probe sets on MOE-430 A/B GeneChip. Three biological replicate array experiments were finished for each condition and MAS5.0 signal were used to do data analysis. Forty-four transcripts with expression differences higher than 2-fold were detected(T test, P<0.05), including Tbx4, Tbx5, Hoxc10 and Pitx1 which were previously shown to be differentially expressed in developing forelimb and hindlimb bud by in situ hybridization and SAGE study (Margulies 2001). RTPCR and in situ experiments confirmed several top differentially expressed genes which were newly discovered by our experiments. Vast amount of

ORGANISM(S): Mus musculus

SUBMITTER: Siming Shou 

PROVIDER: E-GEOD-2560 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress