Expression profiling of yeast kinase and phosphatase knockouts

ABSTRACT: To understand relationships between phosphorylation-based signaling pathways, we analyzed 150 deletion mutants of protein kinases and phosphatases in S. cerevisiae using DNA microarrays. Downstream changes in gene expression were treated as a phenotypic readout. Double mutants with synthetic genetic interactions were included to investigate genetic buffering relationships such as redundancy. Three types of genetic buffering relationships are identified: mixed epistasis, complete redundancy and quantitative redundancy. In mixed epistasis, the most common buffering relationship, different gene-sets respond in different epistatic ways. Mixed epistasis arises from pairs of regulators that have only partial overlap in function and that are coupled by additional regulatory links such as repressi

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Patrick Kemmeren 

PROVIDER: E-GEOD-25644 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress