Development of Laser Capture Microdissection to study the gene expression of the sheep early ovarian folliculogenesis

ABSTRACT: Background Correct achievement of early ovarian folliculogenesis is a crucial phase for further ovarian function. This process is closely regulated by cell-cell interactions and coordinated expression of genes from oocyte and granulosa cells. But, despite of the large number of studies, little is known about the precise gene expression patterns driving early folliculogenesis. The experimental limitations concerned the very small size of these follicles and the mixture of the different developmental stages within an ovary that make the study of isolated follicular components much more difficult. The recently developed laser capture microdissection (LCM) technique coupled with microarrays experiments is promising in addressing the molecular specificity of each follicular compartment. Neverth

ORGANISM(S): Bos taurus

SUBMITTER: Agnes Bonnet 

PROVIDER: E-GEOD-25652 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress