High recombination rates and hotspots in a Plasmodium falciparum genetic cross.

ABSTRACT: We used a high-density tiling array to estimate genetic recombination rate among 32 independent recombinant progeny of a P. falciparum genetic cross (7G8 M-CM-^W GB4). We detected 3184 segregating multi-probe single-feature polymorphisms (mSFPs) and 638 recombination events (496 excluding those from subtelomeric regions). These data, in combination with results from 254 previously reported microsatellites, enabled us to construct a high-resolution genetic map. Comparing genetic and physical maps, we obtained an overall recombination rate of 9.6 kb/cM (12.8 kb/cM excluding subtelomeric regions) and identified 54 hotspots, some of which occurred in genes encoding surface antigens or proteins with repetitive motifs that might play a role in genetic recombination in the parasite. Motifs enrich

ORGANISM(S): Plasmodium falciparum

SUBMITTER: Hongying Jiang 

PROVIDER: E-GEOD-25656 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress