Depletion of eIF4G from yeast cells narrows the range of translational efficiencies genome-wide

ABSTRACT: Investigation of the effects of genetically depleting eIF4G from yeast cells on global translational efficiencies, using gene expresssion microarrays to measure the abundance of mRNA in polysomes relative to total mRNA for ~5900 genes Three biological replicates were examined (designated projects I, II, and III), representing heavy polysomes (HP), light polysomes (LP), and total RNA (T) preparations from three independent pairs of WT and eIF4G degron mutant cultures. Cy3-labeled cDNAs were generated from the 3 HP, 3 LP, and 3 total RNA samples prepared for each strain and the resulting 18 sets of cDNAs were used to probe three (technical) replicate whole-genome microarrays, containing multiple 60-mer oligonucleotides for each gene. The “normalized gene expression summary values” were calcu

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Eun-Hee Park 

PROVIDER: E-GEOD-25721 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress